Bacterial populations exhibit a range of metabolic states influenced by their environment,
intra-and interspecies interactions. The identification of bacterial metabolic states and …

Highlights•Bacterial cell biology has powerful synergies with systems biology.•Single-cell
and single-molecule imaging reveal the robustness of cell-wall growth.•Genetic pathways …

Uptake and excretion of nutrients is an integral part of a cell's physiology. Using analytical
chemistry techniques, metabolite uptake and excretion from the culture medium can be …

Nicotinamide adenine dinucleotide (NADH) is an endogenous fluorescent molecule
commonly used as a metabolic biomarker. Fluorescence lifetime imaging microscopy (FLIM) …

Capturing microbial growth on a macroscopic scale is of great importance to further our
understanding of microbial life. However, methods for imaging microbial life on a scale of …

Bacteria are microorganisms central to health and disease, serving as important model
systems for our understanding of molecular mechanisms and for developing new …

Bacterial pathogens are known for their wide range of strategies to specifically adapt to host
environments and infection sites. An in-depth understanding of these adaptation …

In this review, we discuss multi-color single-molecule imaging and tracking strategies for
studying microbial cell biology. We first summarize and compare the methods in a detailed …

Nanoscopic investigation of bacterial cells is essential to reveal their physiological status,
impacting all cellular functions. Currently, this requires labeled probes or targeted staining …

Bacterial flagella are nanomachines that drive bacteria motility and taxis in response to
environmental changes. Whether flagella are permanent cell structures and, if not, the …