… The enzyme cleaves the LPXTG at the amide bond between the … Studies on the hydrolysis
of the optimal substrate, LPETG ( … Protein engineering approaches might yield Sortase A …

… optimization and application of histidine functionalized … enhance or repress gene expression
by sequence-specific DNA … for the hydrolysis of acyl esters (Ac-LHLHLQL-CONH 2 ). (b, c) …

… ) a direct result of sequestering the amide bonds in the interior of … peptide synthesis for the
first time, we recommend optimizing … In the absence of sequence-specific coupling challenges, …

… the peptide bond in the far UV region (205 nm, 215 nm, 225 nm, and 280 nm). The compound
was ineffective towards Serratia marcescens but active … To acquire the optimal pH of the …

… proteins dramatically, which has provided protein engineers … ) was found to display optimal
properties in terms of activity … intercepted by amines to form amide bonds. Aiming to enhance …

… an active full length protein with the pro-sequence covalently … the robustness of intramolecular
hydrolysis, we cannot fully … using different instrument settings optimized for these mass …

… efforts have been made in protein engineering. For example, … , LPXTG (known as a sortag)
and cleaves the amide bond … is not sequence-specific but distance-dependent for proximity …

… To optimize the splicing efficiency and the regulation of … (B) Sequence-specific sensor for low
concentrations of DNA … to isolate mutants with affinity for Ni 2+ , Zn 2+ , and Cu 2+ . After five …

… enzyme engineering field in their design and optimization for … had enormous impact in
protein engineering with significant … are not limited to protease engineering with tuned substrate …

… ADCs are a challenging class of drugs, since for optimal … some time as a useful protein
engineering tool, allowing the … payload, conjugated via a peptide bond to the C-terminal …