A Salt Bridge and Disulfide Bond within the Lassa Virus Fusion Domain Are Required for the Initiation of Membrane Fusion

HN Pennington, D Birtles, ZW Shi, J Lee - ACS omega, 2024 - ACS Publications
Infection with Lassa virus (LASV), an Old-World arenavirus that is endemic to West Africa,
causes Lassa fever, a lethal hemorrhagic fever. Delivery of LASV's genetic material into the …

Chemical cleavage of an Asp-Cys sequence allows efficient production of recombinant peptides with an N-terminal cysteine residue

K Pane, M Verrillo, A Avitabile, E Pizzo… - Bioconjugate …, 2018 - ACS Publications
Peptides with an N-terminal cysteine residue allow site-specific modification of proteins and
peptides and chemical synthesis of proteins. They have been widely used to develop new …

Bioprocessing of inclusion bodies from E. coli. to prosduce bioactive recombinant proteins

AK Rani, R Katiyar, AS Rathore - Biochemical Engineering Journal, 2023 - Elsevier
Recombinant protein production has revolutionized healthcare with biopharmaceutical
products dominating the pharmaceutical pipelines today. E. coli remains one of the most …

Recombinant expression of HIV-1 protease using soluble fusion tags in Escherichia coli: A vital tool for functional characterization of HIV-1 protease

S Eche, ML Gordon - Virus Research, 2021 - Elsevier
HIV-1 protease expression in the laboratory is demanding because of its high cytotoxicity,
making it difficult to express in bacterial expression systems such as Escherichia coli. To …

[HTML][HTML] Construction, investigation and application of TEV protease variants with improved oxidative stability

E Bayar, Y Ren, Y Chen, Y Hu, S Zhang… - … of Microbiology and …, 2021 - ncbi.nlm.nih.gov
Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type
TEVp is less stable under oxidized redox state. In this work, we introduced and combined …

[HTML][HTML] Identification of Targets of Transcription Factor WRINKLED1-Like Related to Lipid Biosynthesis From Marine Microalga Dunaliella parva

C Shang, B Pang, H Yu, S Gan, Y Li - Frontiers in Marine Science, 2022 - frontiersin.org
WRINKLED1 (WRI1) is an important transcription factor controlling lipid biosynthesis. To
elucidate the function of Dunaliella parva WRI1-like (WRI1-like)(ie, DpWRI1-like), the targets …

Concomitant disorder and high‐affinity zinc binding in the human zinc‐and iron‐regulated transport protein 4 intracellular loop

EM Bafaro, MW Maciejewski, JC Hoch… - Protein …, 2019 - Wiley Online Library
The human zinc‐and iron‐regulated transport protein 4 (hZIP4) protein is the major plasma
membrane protein responsible for the uptake of zinc in the body, and as such it plays a key …

[HTML][HTML] An integrative approach to determine 3D protein structures using sparse paramagnetic NMR data and physical modeling

K Gaalswyk, Z Liu, HJ Vogel… - Frontiers in Molecular …, 2021 - frontiersin.org
Paramagnetic nuclear magnetic resonance (NMR) methods have emerged as powerful tools
for structure determination of large, sparsely protonated proteins. However traditional …

[HTML][HTML] Recombinant Expression and Characterization of α-Conotoxin LvIA in Escherichia coli

X Zhu, J Bi, J Yu, X Li, Y Zhang, D Zhangsun, S Luo - Marine drugs, 2016 - mdpi.com
α-Conotoxin LvIA is derived from Conus lividus, native to Hainan, and is the most selective
inhibitor of α3β2 nicotinic acetylcholine receptors (nAChRs) known to date. In this study, an …

Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus

S Liao, Y Chen, Y Yang, G Wang, Q Wang, J Liu… - Journal of …, 2023 - Elsevier
The RNA synthesis of porcine epidemic diarrhea virus (PEDV) is a sophisticated process
performed by a multilingual viral replication complex, together with cellular factors. A key …