A Salt Bridge and Disulfide Bond within the Lassa Virus Fusion Domain Are Required for the Initiation of Membrane Fusion
HN Pennington, D Birtles, ZW Shi, J Lee - ACS omega, 2024 - ACS Publications
Infection with Lassa virus (LASV), an Old-World arenavirus that is endemic to West Africa,
causes Lassa fever, a lethal hemorrhagic fever. Delivery of LASV's genetic material into the …
causes Lassa fever, a lethal hemorrhagic fever. Delivery of LASV's genetic material into the …
Chemical cleavage of an Asp-Cys sequence allows efficient production of recombinant peptides with an N-terminal cysteine residue
Peptides with an N-terminal cysteine residue allow site-specific modification of proteins and
peptides and chemical synthesis of proteins. They have been widely used to develop new …
peptides and chemical synthesis of proteins. They have been widely used to develop new …
Bioprocessing of inclusion bodies from E. coli. to prosduce bioactive recombinant proteins
AK Rani, R Katiyar, AS Rathore - Biochemical Engineering Journal, 2023 - Elsevier
Recombinant protein production has revolutionized healthcare with biopharmaceutical
products dominating the pharmaceutical pipelines today. E. coli remains one of the most …
products dominating the pharmaceutical pipelines today. E. coli remains one of the most …
Recombinant expression of HIV-1 protease using soluble fusion tags in Escherichia coli: A vital tool for functional characterization of HIV-1 protease
HIV-1 protease expression in the laboratory is demanding because of its high cytotoxicity,
making it difficult to express in bacterial expression systems such as Escherichia coli. To …
making it difficult to express in bacterial expression systems such as Escherichia coli. To …
[HTML][HTML] Construction, investigation and application of TEV protease variants with improved oxidative stability
E Bayar, Y Ren, Y Chen, Y Hu, S Zhang… - … of Microbiology and …, 2021 - ncbi.nlm.nih.gov
Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type
TEVp is less stable under oxidized redox state. In this work, we introduced and combined …
TEVp is less stable under oxidized redox state. In this work, we introduced and combined …
[HTML][HTML] Identification of Targets of Transcription Factor WRINKLED1-Like Related to Lipid Biosynthesis From Marine Microalga Dunaliella parva
C Shang, B Pang, H Yu, S Gan, Y Li - Frontiers in Marine Science, 2022 - frontiersin.org
WRINKLED1 (WRI1) is an important transcription factor controlling lipid biosynthesis. To
elucidate the function of Dunaliella parva WRI1-like (WRI1-like)(ie, DpWRI1-like), the targets …
elucidate the function of Dunaliella parva WRI1-like (WRI1-like)(ie, DpWRI1-like), the targets …
Concomitant disorder and high‐affinity zinc binding in the human zinc‐and iron‐regulated transport protein 4 intracellular loop
EM Bafaro, MW Maciejewski, JC Hoch… - Protein …, 2019 - Wiley Online Library
The human zinc‐and iron‐regulated transport protein 4 (hZIP4) protein is the major plasma
membrane protein responsible for the uptake of zinc in the body, and as such it plays a key …
membrane protein responsible for the uptake of zinc in the body, and as such it plays a key …
[HTML][HTML] An integrative approach to determine 3D protein structures using sparse paramagnetic NMR data and physical modeling
Paramagnetic nuclear magnetic resonance (NMR) methods have emerged as powerful tools
for structure determination of large, sparsely protonated proteins. However traditional …
for structure determination of large, sparsely protonated proteins. However traditional …
[HTML][HTML] Recombinant Expression and Characterization of α-Conotoxin LvIA in Escherichia coli
X Zhu, J Bi, J Yu, X Li, Y Zhang, D Zhangsun, S Luo - Marine drugs, 2016 - mdpi.com
α-Conotoxin LvIA is derived from Conus lividus, native to Hainan, and is the most selective
inhibitor of α3β2 nicotinic acetylcholine receptors (nAChRs) known to date. In this study, an …
inhibitor of α3β2 nicotinic acetylcholine receptors (nAChRs) known to date. In this study, an …
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus
S Liao, Y Chen, Y Yang, G Wang, Q Wang, J Liu… - Journal of …, 2023 - Elsevier
The RNA synthesis of porcine epidemic diarrhea virus (PEDV) is a sophisticated process
performed by a multilingual viral replication complex, together with cellular factors. A key …
performed by a multilingual viral replication complex, together with cellular factors. A key …