… Native-like proteins are then obtained only upon optimization … in a much more
sequence-specific manner. Such structures … the formation and hydrolysis of peptide bonds. In …

… The methods of protein engineering to design artificial … Substrate and sequence specific
hydrolytic cleavage with model … of N-(2-aminoethyl)-glycine units linked by peptide bonds. As a …

… question being actively addressed by protein design. Here, … structural and hydrolytic dual-site
metalloprotein (Section 4.1). … which, upon binding Ni II , Co II , or Ru II , self-assembles to …

… ADARYKS that was shown to have modest, sequence specific … in both the identification and
optimization of peptide structures. In … barriers for peptide bond formation and hydrolysis are …

… forge backbone amide bonds on recombinant proteins, whose … of sequence specific proteases
to liberate cysteinyl proteins … Consensus protein engineering, wherein multiple sequence …

… sequence-specific redox reactivity of cysteine residues. To this end, a solid-supported
combinatorial peptide … ,3-diketone derivatives93 or amide bond formation with β-lactam derivatives.…

… domains that hybridize with a sequence-specific domain of an … , the need to optimize the
sequences associated with the different … In a further study, the Ni 2+ -dependent hydrolytic RNA-…

… dependent catalysis does not require the formation of a stable complex. Instead, metal ions
can enter and exit an active … may include the peptide bond hydrolysis in X1motifs by released …

… level of sequence identity with respect to the active site and … peptide of mNisA, initial activity
tests and optimizations of the … hydrolysis of the unmodified and dehydrated peptides. As …

… To further optimize switch performance, an in vivo directed … -Tu to hydrolyze GTP and release
tRNA into the ribosomal A-site. 2) Peptide bond formation occurs, transferring the peptide to …