An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells

P Stojkovic, M Lako, R Stewart, S Przyborski… - Stem Cells, 2005 - academic.oup.com
Human embryonic stem cells (hESCs) have great potential as a source of cells for
therapeutic uses, but their culture requires the support of mouse or human cells, either …

[HTML][HTML] Characterization of integrin engagement during defined human embryonic stem cell culture

Y Meng, S Eshghi, YJ Li, R Schmidt… - The FASEB …, 2010 - ncbi.nlm.nih.gov
Human embryonic stem (hES) cells are pluripotent, capable of differentiating into any cell
type of the body, and therefore have the ability to provide insights into mechanisms of …

A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid …

ES Ng, R Davis, EG Stanley, AG Elefanty - Nature protocols, 2008 - nature.com
In order to promote the uniform and reproducible differentiation of human embryonic stem
cells (HESCs) in response to exogenously added growth factors, we have developed a …

Long‐term culture of human embryonic stem cells in feeder‐free conditions

ES Rosler, GJ Fisk, X Ares, J Irving… - … dynamics: an official …, 2004 - Wiley Online Library
We have demonstrated previously that human embryonic stem (hES) cells possess a
characteristic morphologic, antigenic, and molecular profile that can be used to assess the …

Embryonic stem cells

A Biswas, R Hutchins - Stem cells and development, 2007 - liebertpub.com
Stem cells are unique cell populations with the ability to choose between self-renewal and
differentiation. Embryonic stem (ES) cells have the ability to form any fully differentiated cell …

Methods for culturing mouse and human embryonic stem cells

S Lin, P Talbot - Embryonic Stem Cell Therapy for Osteo-Degenerative …, 2011 - Springer
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 years ago
and ever since have been valuable tools for creating knockout mice and for studying early …

Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium

C Xu, E Rosler, J Jiang, JS Lebkowski, JD Gold… - Stem …, 2005 - academic.oup.com
Previous studies have shown that prolonged propagation of undifferentiated human
embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders …

Basic fibroblast growth factor support of human embryonic stem cell self-renewal

ME Levenstein, TE Ludwig, RH Xu, RA Llanas… - Stem …, 2006 - academic.oup.com
Human embryonic stem (ES) cells have most commonly been cultured in the presence of
basic fibroblast growth factor (FGF2) either on fibroblast feeder layers or in fibroblast …

Extracellular matrix-mediated differentiation of human embryonic stem cells: differentiation to insulin-secreting beta cells

K Narayanan, VY Lim, J Shen, ZW Tan… - … Engineering Part A, 2014 - liebertpub.com
Stem cells have tremendous potential for treating various human diseases. Protocols have
been established to differentiate stem cells into specific lineages through the provision of …

Self-renewal vs. differentiation of mouse embryonic stem cells

KS O'Shea - Biology of reproduction, 2004 - academic.oup.com
Embryonic stem (ES) cells are typically derived from the inner cell mass of the
preimplantation blastocyst and can both self-renew and differentiate into all the cells and …