… two angles phi and psi which form around the peptide bonds … of the unsolved challenges in
protein engineering.14,15 Design … optimization of the internal binding site, we designed two …

… procedure depend mainly on the level of optimization which … The AAs forming cleaved
peptide bond in a substrate are … involved in its inactivation (hydrolytic cleavage) within the neural …

… for pHis due to the rapid hydrolysis of the phosphoramidate bond and the spontaneous …
pHis sequence-specific antibodies. Improved antibodies together with optimized techniques (40–…

… , and optimal positioning will depend on the individual protein … metal ions like Cu 2+ , Co
2+ , Zn 2+ , or Ni 2+ , which are … Polypeptide sequence-dependent hydrolysis by Nickel (Ni) …

… presence of highly repetitive peptide sequences, and to the … partial hydrolysis treatment
and obtained their sequences with … Thus, their sequences are evolutionarily optimized for very …

… peptide bond in proteins or the phosphodiester bond in DNA. It possesses robust biological
properties that make it resistant to hydrolysis by … orientation of the antibody active site and the …

… considered to choose an optimal modification method. Among … efforts have been made in
protein engineering. For example, … , LPXTG (known as a sortag) and cleaves the amide bond …

… Nature has evolved an optimal synthetic factory in the form of … by the complexation of Ni 2+
ions to proteins embedded with … labeling by sequence-specific assembly through capitalizing …

… commercial value, protein engineering of biosynthetic … downstream activated substrates via
a peptide bond. C-domains … of the protein and establishes sequence-specific DNA-binding, …

… Optimal synthesis of H2ases requires either strict anaerobiosis … of the dimeric protein that
allows sequencespecific DNA binding … will be important in directing protein engineering, eg in …