… When the amide bond between the last regular amino acid … the creation and optimization of
any type of peptide sequence to … metal ions, such as: Hg 2+ , Pb 2+ or Ni 2+ [180]. In general, …

… two angles phi and psi which form around the peptide bonds … of the unsolved challenges in
protein engineering.14,15 Design … optimization of the internal binding site, we designed two …

… Optimal synthesis of H2ases requires either strict anaerobiosis … of the dimeric protein that
allows sequencespecific DNA binding … will be important in directing protein engineering, eg in …

… products that resulted from partial acid hydrolysis of the native peptides. Combined utilization
of HPLC, fast atom bombardment mass spectrometry and peptide recognition software (“F-…

… for forming the amide bond in protein synthesis catalyzed by the … Modern protein engineering
methods allow for the … a new variant of the Jacobsen hydrolytic kinetic resolution to access …

… A: sequence specific for GTP; B: sequence specific for … only those that form ether bridges or
amide bonds are acceptable. … Two main avenues are open for this optimization: the first is the …

… , catalyzes the hydrolysis of α-1, 4-glycosidic bonds, leading … gene (epl M ) by using
sequence-specific primers (F 5′ catatg ATG … alkaline pH conditions for optimal catalytic …

… to a site-or sequence-specific mechanism. The extracellular … (DH), defined as the percentage
of cleaved peptide bonds, is … in recent studies to optimize hydrolysis conditions including …

… Appropriate modification regulation and sequence optimization … By wrapping Cre mRNA and
sequence-specific DNA … molecule from an ester bond (O series) to an amide bond (N series)…

… inexpensive, easy to modify and can be sequence-specific. In … An optimal probe should have
the following characteristics: 1… Unlike many other metal ions, Ni 2+ and Co 2+ also quench …