Method of counting microorganisms and device for accomplishing the counting

T Takahashi - US Patent 6,385,272, 2002 - Google Patents
A filter on which living bacteria are captured is processed with an extraction reagent and a
luminescence reagent. The state of luminescence of the filter is photographed by a …

Co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy

RH Singer, C Eliscovich, SM Shenoy - US Patent App. 16/476,582, 2019 - Google Patents
US20190339204A1 - Co-localization at molecular resolution of multiple fluorescence
channels acquired using optical microscopy - Google Patents US20190339204A1 - Co-localization …

Fluorescence lifetime imaging microscopy

CW Chang, D Sud, MA Mycek - Methods in cell biology, 2007 - Elsevier
Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially
resolved images of fluorophore lifetime (the property describing how rapidly fluorescence …

Live cell fluorescence microscopy to observe essential processes during microbial cell growth

M Howell, JJ Daniel, PJB Brown - JoVE (Journal of Visualized …, 2017 - jove.com
Core cellular processes such as DNA replication and segregation, protein synthesis, cell
wall biosynthesis, and cell division rely on the function of proteins which are essential for …

Developing a novel fluorescence lifetime imaging microscope with applications to sensing metabolic function and oncogene activity in vivo.

W Zhong - 2005 - elibrary.ru
In contrast to intensity-based fluorescence microscopy, fluorescence lifetime imaging
microscopy (FLIM) bases image contrast on fluorophore excited-state lifetime. The …

Method and system for characterizing a sample by imaging fluorescence microscopy

A Meixner, F Schleifenbaum - US Patent App. 13/144,729, 2011 - Google Patents
A method for characterizing a sample by imaging fluorescence microscopy includes
detecting fluorescence intensity in a time-resolved fashion after switching off excitation …

[HTML][HTML] The design of simple bacterial microarrays: Development towards immobilizing single living bacteria on predefined micro-sized spots on patterned surfaces

NB Arnfinnsdottir, V Ottesen, R Lale, M Sletmoen - PloS one, 2015 - journals.plos.org
In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy,
and applicable in a standard molecular biology laboratory. Microcontact printing is used to …

Method for the detection of dyes in fluorescence microscopy

R Wolleschensky, G Moehler - US Patent 6,958,811, 2005 - Google Patents
(57) ABSTRACT A method for optical detection of characteristic quantities of the wavelength-
dependent behavior of an illuminated Specimen, Such as the emission behavior and/or …

Use of digitized video microscopy with a fluorogenic enzyme substrate to demonstrate cell‐and compartment‐specific gene expression in Salmonella enteritidis and …

PJ Lewis, CE Nwoguh, MR Barer… - Molecular …, 1994 - Wiley Online Library
A rapid and sensitive method for detection of cell‐and compartment‐specific gene
expression in individual cells of both Gram‐negative and Gram‐positive microorganisms is …

Imaging Mycobacterium tuberculosis in mice with reporter enzyme fluorescence

R Sharan, HJ Yang, P Sule, JD Cirillo - JoVE (Journal of Visualized …, 2018 - jove.com
Reporter enzyme fluorescence (REF) utilizes substrates that are specific for enzymes
present in target organisms of interest for imaging or detection by fluorescence or …